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Compatibility
Check path (14 rules)
Structure
Molecular architecture — a violation blocks expression
N/A
N/A
N/A
N/A
N/A
N/A
Suggestions
Restriction sites in CAR CDS Partial
Topology
Domain arrangement & positional heuristics
N/A
N/A
N/A
N/A
Suggestions
Redundant signal peptides Planned
CD28-TM + CD28 costimulation Research
Function
Functional minimum requirements for CAR-T activation
N/A
N/A
Suggestions
Backbone capacity (insert size) Planned
GC content of the CAR sequence Planned
Clinical
Clinical recommendations — based on clinical literature
N/A
N/A
Suggestions
Patent status (FMC63, BCMA …) Research
WPRE / promoter orientation Planned
Legend
Compliant
Error
Warning
Note
Ignored
N/A (empty)
Rule title is clickable → jumps to the flag card (list).
Note: All checks can be ignored manually (scientific innovation). Ignored errors allow the full analysis but are flagged in the report. Heuristics (<100% confidence) are literature-based recommendations, not blockers.